Protein A Agarose Beads from Cell Signaling Technology

Product Specs
ItemProtein A Agarose Beads
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9863P
Quantity200 µl
ApplicationsIP
Research AreaRelated Products
TypePrecast Gels / Media / Resins / Beads, Resins, Beads
ModeAntibody Purification

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Zika virus remodelled ER membranes contain proviral factors involved in redox and methylation pathwaysNat CommunDecember 5, 2023Solène Denolly, Alexey Stukalov, Uladzimir Barayeu, Alina N. Rosinski, Paraskevi Kritsiligkou, Sebastian Joecks, Tobias P. Dick, Andreas Pichlmair, Ralf BartenschlagermM Tris-HCl [pH 7.4], 150 mM NaCl, 0.1% NP-40 and 10U RNase inhibitor), Samples were incubated with protein A agarose beads at 4 °C for 2 h. After 3 washes with IP buffer, bound RNAs were eluted with 2×100 uL of elution buffer

(2) Spinal microRNA-134-5p targets glutamate receptor ionotropic kainate 3 to modulate opioid induced hyperalgesia in miceMolecular PainDecember 1, 2023Zhen Wang, Yao Yao, Yuzhu Tao, Peixin Fan, Yonghao Yu, Keliang Xie, Guolin WangArgonaute-2 antibodies (1:50, Cell Signaling Technology C34C6) at 4°C overnight. On the following day, protein A agarose beads (Santa Cruz Biotechnology) were added, mixed, incubated for 1 h at 4°C, and then centrifuged. The supernatant

(3) The survivin-ran inhibitor LLP-3 decreases oxidative phosphorylation, glycolysis and growth of neuroblastoma cellsBMC CancerNovember 25, 2023Celimene Galiger, Fatema Tuj Zohora, Carmen Dorneburg, Daniel Tews, Klaus-Michael Debatin, Christian Beltinger(10,508–1-AP, Proteintech) and 2–4 µg rabbit IgG (2729, Cell Signaling Technology), and with 30 μl of protein A agarose beads (16–125, Merck Millipore) overnight at 4 °C. For Western blot analysis of OXPHOS and glycolysis proteins

(4) MIWI N-terminal RG motif promotes efficient pachytene piRNA production and spermatogenesis independent of LINE1 transposon silencingPLOS GeneticsNovember 13, 2023Chao Wei, Jiongjie Jing, Xiaoyuan Yan, Jeffrey M. Mann, Ruirong Geng, Huirong Xie, Elena Y. Demireva, Rex A. Hess, Deqiang Ding, Chen Chen4°C. Anti-MILI (PM044, MBL) or anti-MIWI (2079, Cell Signaling Technology) antibodies together with protein-A agarose beads were added to the lysates and incubated for 4 h at 4°C. The beads were washed in lysis buffer 5 times.

(5) SUMOylation indirectly suppresses activity of the HIF-1α pathway in intestinal epithelial cellsJournal of Biological ChemistryNovember 1, 2023Mykyta I. Malkov, Darragh Flood, Cormac T. Taylorisolate the antibody-bound proteins, 20 μl of Protein A Agarose Beads (Cell Signaling; catalog no.: 9863) were added to each sample and incubated for 4 h at 4 °C with end-to-end rotation. Agarose beads were

(6) Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/β-catenin signalingBone researchOctober 26, 2023Liu RX, Gu RH, Li ZP, Hao ZQ, Hu QX, Li ZY, Wang XG, Tang W, Wang XH, Zeng YK, et al.protease inhibitors. After incubation on ice for 30 min, 40 μL of 50% protein A agarose bead slurry (CST, Cat# 9863, USA) was used to preclear cell extracts for 30 min at 4 °C, followed by incubation with antibodies

(7) Single-cell signaling analysis reveals that Major Vault Protein facilitates RasG12C inhibitor resistancebioRxiv : the preprint server for biologyOctober 4, 2023Zhang JZ, Ong SE, Baker D, Maly DJ.Fisher,PI26620 Spectra™ Multicolor High Range Protein Ladder,Thermo Fisher,26625 Protein A Agarose Beads,CST,9863S Protein G Agarose Beads,CST,37478 Puromycin,Tocris,4089 Hygromycin,Tocris,4137 DPBS,Gibco,14190250 Doxycycline,Tocris,4090 PEI

(8) Raptor mediates the selective inhibitory effect of cardamonin on RRAGC-mutant B cell lymphomaBMC Complementary Medicine and TherapiesSeptember 26, 2023Ying Liu, Yanting Zhu, Huajiao Chen, Jintuo Zhou, Peiguang Niu, Daohua ShiHRP-labeled anti-mouse (#7076) and anti-rabbit (#7074) secondary antibody, Protein A Agarose Beads (#9863), Rabbit IgG Isotype Control (#3900) were from Cell Signaling Technology (Danvers, MA, USA). Cardamonin

(9) Dual Targeting of Apoptotic and Signaling Pathways in T-Lineage Acute Lymphoblastic LeukemiaClinical Cancer ResearchAugust 15, 2023Caner Saygin, Giorgia Giordano, Kathryn Shimamoto, Bart Eisfelder, Anika Thomas-Toth, Girish Venkataraman, Vijayalakshmi Ananthanarayanan, Tiffaney L. Vincent, Adam DuVall, Anand A. Patel, et al.and incubated with rotation for 2 hours at 4°C. Protein A agarose beads (Cell Signaling Technology, #9863) were added to lysates (10–30 μL of 50% bead slurry), which were subsequently incubated with rotation

(10) Mitotic phosphorylation of Tau/MAPT modulates cell cycle progression in prostate cancer cellsJournal of Cancer Research and Clinical OncologyAugust 1, 2023Letizia Clementi, Samantha Sabetta, Veronica Zelli, Chiara Compagnoni, Alessandra Tessitore, Vincenzo Mattei, Adriano AngelucciTechnology) was incubated for 2 h at 4 °C with protein-G Agarose Beads (Cell Signalling Technology) and Protein A-Agarose Beads (Santa Cruz Biotechnology) in Tris 50 mM pH 7.4, NP40 1%, Na-deoxycholate 0.25%, NaCl 150 mM and EDTA

(11) LAR Downregulation Protects the Astrocytic U251 and Cocultured SH-SY5Y Cells in a Rotenone-Induced Parkinson’s Disease Cell ModelInternational Journal of Molecular SciencesJuly 5, 2023Wei Zheng, Xiao Han, Bing Han, Gang Li, Jing Gan, Tian Wang, Bo Xu, Jie He, Wenxiao Du, Xiaolin Cao, et al.protein using anti-IGF-1Rβ (Cell Signaling Technology, Danvers, MA, USA) followed by incubation with protein A agarose beads (Absin, Shanghai, China). Samples were separated via 5%, 6%, 10%, 12%, or 15% gels SDS-PAGE (Mini-PROTEAN,

(12) AdipoR2 recruits protein interactors to promote fatty acid elongation and membrane fluidityJournal of Biological ChemistryJune 1, 2023Mario Ruiz, Ranjan Devkota, Delaney Kaper, Hanna Ruhanen, Kiran Busayavalasa, Uroš Radović, Marcus Henricsson, Reijo Käkelä, Jan Borén, Marc Pilonmonoclonal anti-HA antibody, 7 μl (C29F4, Cell signaling, RRID: AB_1549585, Cat# 3724) and 20 μl of protein A-agarose beads (Roche) were added to the lysate, which was then rotated at 4 °C for 1 h (samples for mass spectroscopy).

(13) Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assemblyJournal of CancerMay 21, 2023Jintian Song, Yigui Chen, Hui Yu, Liang Zheng, Yi Wang, Dan Liinhibitor (36978, Thermo, USA). Subsequently, extracted protein and 50 mL Protein A Agarose Beads (9863, CST, USA) were fully reacted for 4 h at room temperature, and then the admixture was incubated with

(14) Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signalingMolecular CellMay 18, 2023Edoardo José Longarini, Helen Dauben, Carolina Locatelli, Anne R. Wondisford, Rebecca Smith, Charlotte Muench, Andreas Kolvenbach, Michelle Lee Lynskey, Alexis Pope, Juan José Bonfiglio, et al.65601 Chromotek GFP-trap Magnetic Particles M-270,Chromotek,Cat# gtd Protein A Agarose Beads,Cell Signaling,Cat# 9863 "Pierce™ NeutrAvidin™ Coated Plates, Black, 96-Well",Themo Scientific,Cat# 15117 Guide-it CRISPR/Cas9

(15) Inhibition of ERK signaling for treatment of ERRα positive TNBCPLOS ONEMay 10, 2023David Musheyev, Esther Miller, Natania Birnbaum, Elisheva Miller, Shoshana Erblich, Alyssa Schuck, Anya Alayeveither Sepharose®Bead Conjugated p44/42 MAPK (Erk1/2) antibody #5736 (Cell Signaling Technology) or Protein A Agarose beads, 50% slurry (EMD Millipore) overnight at 4°C. Beads were pelleted from solution, washed twice with IP

(16) Driver Mutations Dictate the Immunologic Landscape and Response to Checkpoint Immunotherapy of GlioblastomaCancer Immunology ResearchMay 3, 2023Alan T. Yeo, Rushil Shah, Konstantinos Aliazis, Rinku Pal, Tuoye Xu, Piyan Zhang, Shruti Rawal, Christopher M. Rose, Frederick S. Varn, Vicky A. Appleman, et al.NaCl). A phospho-tyrosine specific antibody (P-Tyr-1000, Cell Signaling Technology) was incubated with protein A agarose beads (Roche) overnight at 4°C in 1% PBS to bind the antibody to the beads. Subsequently, the antibody-bead

(17) RBM14 as a novel epigenetic-activated tumor oncogene is implicated in the reprogramming of glycolysis in lung cancerWorld Journal of Surgical OncologyApril 14, 2023Yan Hu, Hanshuo Mu, Zhiping Deng10 min, the cell lysates were incubated with indicated antibody and protein A/G PLUS agarose beads (#9863, #37478, Cell Signaling Technology) overnight at 4 °C. After washing, the beads were lysed with loading

(18) CDK9 inhibition induces epigenetic reprogramming revealing strategies to circumvent resistance in lymphomaMolecular CancerMarch 30, 2023Elana Thieme, Nur Bruss, Duanchen Sun, Edward C. Dominguez, Daniel Coleman, Tingting Liu, Carly Roleder, Melissa Martinez, Krystine Garcia-Mansfield, Brian Ball, et al.pre-cleared and incubated at 4 °C overnight with 2 µg of BRD4 antibody or rabbit IgG isotype control. Protein A agarose beads (20 µL, Cell Signaling Technology) were added to lysates and samples were incubated for 1.5 h at 4 °C.

(19) Bag-1 Protects Nucleus Pulposus Cells from Oxidative Stress by Interacting with HSP70BiomedicinesMarch 12, 2023Suyama K, Sakai D, Hayashi S, Qu N, Terayama H, Kiyoshima D, Nagahori K, Watanabe M.Germany) and PhosSTOPTM (Roche). Immunoprecipitation was performed using Protein A Agarose Beads (#9863; Cell Signaling Technology) following a standard protocol. Proteins were separated by sodium dodecyl

(20) Linear ubiquitination of LKB1 activates AMPK pathway to inhibit NLRP3 inflammasome response and reduce chondrocyte pyroptosis in osteoarthritisJournal of orthopaedic translationMarch 1, 2023Chen Y, Liu Y, Jiang K, Wen Z, Cao X, Wu S.Proteintech, USA) at 4 °C overnight. Cell lysates were then combined with protein A/G agarose beads (#9863, Cell Signaling Technology, USA) for 2 h. Subsequently, collected precipitates were washed with ice

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Protein A Agarose Beads from Cell Signaling Technology

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